: Uses a stabilized power supply to minimize output voltage variations, improving reproducibility.
The is a cornerstone in laboratory pipette fabrication, renowned for its ability to produce consistent, high-quality micropipettes for applications like patch clamping and microinjection . Although Narishige has transitioned to the PC-100 , the PC-10 remains a workhorse in many labs worldwide.
Avoid installation near air conditioning vents, windows, or doors, as drafts cause temperature fluctuations in the heating coil. Operate within a stable ambient temperature range ( 20∘C20 raised to the composed with power C 25∘C25 raised to the composed with power C Safety Warnings
, the PC-10 remains a robust tool for creating tips ranging from sub-micron sizes to tens of microns. Core Functionality and Modes
To help refine these instructions for your specific lab setup, let me know:
Adjust the dial to a slightly lower or higher setting depending on the desired taper smoothness.
To avoid burning out the nichrome coil, perform a test pull to find the minimum heat required to melt the glass: Load a standard capillary tube. Set the heater dial to a low value (e.g., 50).
The is a vertical dual-stage glass micropipette puller widely recognized for its versatility and straightforward gravity-based pulling method. Performance Review Summary Pros :
These settings allow the user to display and adjust the specific heating values for each pulling stage on the digital LED readout. Operational Parameters
: Ensure the instrument is perfectly horizontal to maintain pull symmetry. Heater Block Adjustment
The Narishige PC-10 is a precision instrument widely recognized in the fields of neuroscience, physiology, and cell biology. It is a designed specifically for the fabrication of glass microelectrodes and injection pipettes. Unlike horizontal pullers that use a heating filament and gravity, the PC-10 utilizes a vertical design with a weight-based gravitational pull mechanism.
The PC-10 has been a workhorse in laboratories for decades, producing essential tools for critical research techniques.
The Narishige PC-10 manual provides step-by-step instructions on how to operate the device:
The updated Narishige PC-10 manual includes the following new features: